Contact Denis Bragin PhD, DBragin@salud.unm.edu.
Two multi-photon excitation laser scanning microscope-electrophysiology workstations (Workstations 1 and 2) share a SpectraPhysics diode laser pumped Ti-Sapphire laser as their excitation source. These stations can be run simultaneously.
Workstation 1 uses a Bio-Rad Radiance 2000 scanner mounted on an Olympus upright microscope and it is able to carry out in vitro measurements of cellular activity, e.g., synapse function, Ca signaling, electrophysiology, and cell motility. It is compatible with tissue slice or explants.
Workstation 2 uses a Prairie Technologies Ultima scanner mounted on a modified Olympus microscope and is compatible with use for small, anesthetized, animals. To date, projects on brain microcirculation, energy intermediates, and synapse function have been carried out.
Workstation 3 is based around a cooled CCD detection camera and a fast monochrometer for standard fluorescence excitation. These are used in conjunction with a modified upright microscope which will accommodate either small animal or in vitro experiments is the working platform. Near infrared illumination is also available which facilitates patch electrode electrophysiology ex vivo preparations.
Workstation 4 is an inverted microscope – cooled CCD fluorescence system with monochromatic excitation. It is compatible with measurements on cultured cells which can be maintained under sterile conditions with temperature control. Phase contrast illumination is also available.